FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001963535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246269
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.9717016758097852No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20340.8259261214363156No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.7268474716671607No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.4555993649220974No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.3037329099480649No Hit
CTTATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTG5650.22942392262119876No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5360.21764818146011072No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.20018759973849734No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.1981572995383097No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4750.19287851901782196No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGA4520.18353913809695901No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3630.14739979453361973No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC3530.14333919413324453No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC3290.13359375317234407No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT3110.12628467245166872No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.12506649233155614No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT3050.12384831221144359No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC2970.12059983189114341No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2810.11410287125054311No Hit
GTATCAACGCAGAGTACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTC2770.11247863109039302No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG2670.10841803069001783No Hit
ATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGTC2610.10598167044979272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.10557561040975519No Hit
GGGGAGCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAG2600.10557561040975519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)