FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT120712.074829931972788No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2682.681072428971589No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2382.380952380952381No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2252.250900360144058Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2092.0908363345338135No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT1501.5006002400960383Illumina PCR Primer Index 10 (95% over 24bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1401.400560224089636No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG1021.0204081632653061No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.8003201280512205No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC720.7202881152460985No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.6402561024409764No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC530.5302120848339336No Hit
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT430.430172068827531No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTG390.39015606242497Illumina PCR Primer Index 10 (96% over 29bp)
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.350140056022409No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAACTCGTATGCCGT330.33013205282112845No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTACCTCGTATGCCGT320.3201280512204882No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCT260.26010404161664663Illumina PCR Primer Index 10 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT250.2501000400160064Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTAGCTCGTATGCCGT250.2501000400160064No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTG180.18007202881152462Illumina PCR Primer Index 10 (96% over 25bp)
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.18007202881152462No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCC170.17006802721088435No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1600640256102441No Hit
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT150.15006002400960386Clontech Universal Primer Mix Long (95% over 23bp)
GTCTTTGTTTGAGTACTTTTGTTAAGGTTCGTTTTGTGTCGTTGGCGGTG140.1400560224089636No Hit
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13005202080832332No Hit
GTTTTGTGTCGTTGGCGGTGTTTTTTCGCTTTGTTCGTTGGTTCATCCGG130.13005202080832332No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA120.12004801920768307No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.11004401760704283No Hit
CGTATAAGACGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11004401760704283No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCTGCTTGA110.11004401760704283Illumina PCR Primer Index 10 (96% over 29bp)
GAGCGCCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGC110.11004401760704283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC110.11004401760704283No Hit
AACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10004001600640257No Hit
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTA100.10004001600640257No Hit
GTATCAACGCAGAGTACGGGGGAAGAAGGGGGACCGATGTTGGTGAGCAC100.10004001600640257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)