FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9996
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTG1801.800720288115246No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.8403361344537815No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.7703081232492998No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.7202881152460985No Hit
ATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGTC680.6802721088435374No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.4101640656262505No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.2801120448179272No Hit
CATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGTCGCCGT280.2801120448179272Illumina RNA PCR Primer (96% over 25bp)
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.24009603841536614No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1600640256102441No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA110.11004401760704283No Hit
TATACACATCTGACGCTGCCGACGATAAGGCTCGTGTAGATCTCGGTGGT100.10004001600640257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)