FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2429158
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262631.0811565159614978No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT261411.0761341995868527No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243851.003845777014093No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132700.5462798220618009No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86370.35555529940827235No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58870.24234734834045374No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT51000.20994929107122715No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50240.20682063496898925No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC36110.14865233138396103No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC35580.14647050541792672No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT34370.14148935557094267No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA31020.12769856880449934No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA30650.1261754072810414No Hit
CCTTGGGCCAGCTTGGTTTTACTCTAGATTTCACTGTCGTCCCACCCCAC28890.11893009841270102No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27070.11143779037839449No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT26780.11024396107622476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)