FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93110
Sequences flagged as poor quality0
Sequence length151
%GC35

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16791.8032434754591344No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14081.5121898829341638No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9871.0600365159488776No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.807646869294383No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.5251852647406294No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.48007732789174096No Hit
CTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTG4280.459671356460101No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.3436795188486736No Hit
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.3426055203522715No Hit
GTATCAACGCAGAGTACGGGGACCATGCCGGCCAGAACCTGTTGGATCAA2020.21694769627322522No Hit
ATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTC2010.2158736977768231No Hit
AAAACAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.17828375040274944No Hit
CATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGT1530.16432176994952205Illumina Single End PCR Primer 1 (96% over 25bp)
AAAACAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.153581784985501No Hit
GGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGTCCCCGTACT1330.14284180002147998No Hit
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.13424981205026312No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.1288798195682526No Hit
AAATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130.12136183009343786No Hit
AAATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.11706583610782945No Hit
CCGCGAGATGGATTGGGGTTGTTGATCCAACAGGTTCTGGCCGGCATGGT950.10202985715819998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)