FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933086
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1055555.460439939040477No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1043085.395931686432989No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT735993.8073319034952404No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT698383.6127725305547713No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT462622.3931682294528023No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325941.6861122578095336No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267451.3835390665495484No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223501.1561823943683829No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT220891.1426806670784435No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCT197041.0193028142565825Illumina PCR Primer Index 7 (95% over 22bp)
GTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116850.6044738826932687No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCT63510.32854203072186133Illumina PCR Primer Index 7 (96% over 25bp)
CAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.32693837728895664No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTGCTTG61610.3187131871008326Illumina PCR Primer Index 7 (96% over 27bp)
ACTCTGCGTTGATACCACTGCTTAAACGCAGAGTCGACTTTTTTTTTTTT51140.26455108567337404Clontech Universal Primer Mix Long (95% over 23bp)
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT48460.25068724309213347No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC43770.22642551857496251RNA PCR Primer, Index 41 (95% over 22bp)
TCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.21530340605643No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39210.2028362938844935No Hit
AACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.17759168500521963No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34230.17707437744621812No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTCTG34210.17697091593441783RNA PCR Primer, Index 41 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT30110.15576130601535576No Hit
GTATCAACGCAGAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26650.13786246447390338No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG26530.13724169540310158No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC21370.11054862535862346No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT21360.11049689460272331No Hit
CGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.11003131779962194No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21090.10910016419341922No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20890.1080655490754162No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCACCTCGTATGCCGT20610.10661708791021196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)