FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718988
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC77030.448112494095363No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA76540.4452619797229533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT69490.4042494770178733No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT65260.37964197539482536No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC65250.3795838016321231No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC64510.37527894319215727No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC60870.3541036935685415No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG60250.35049692028100254No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC52450.3051213853732545No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA50290.2925558526295704No Hit
GTATAAAACACTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA49760.28947264320635163No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.27411476985295996No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44370.2581169851098437No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43250.2516015236871927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43040.25037987467044565No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41990.2442716295867103No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGTT41850.24345719690887896No Hit
CCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGGCTGCACCA41560.24177015779051395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT41400.24083937758727808No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC39510.22984453643655453No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC37970.2208857769804094No Hit
GGACACGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATC37010.21530109576099427No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36640.21314866654101133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36230.21076354227021946No Hit
GTACTCTATCGATCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGA36080.20989093582968582No Hit
GTATTATAGTACTCTATCGATCACCTTCGGCCAAGGGACACGACTGGAGA35890.20878563433834327No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA35810.20832024423672532No Hit
GGTTAGGAGGCTGTCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTA35190.20471347094918635No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35100.20418990708486623No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACTGCCGGGT34860.20279373678001242No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC33570.19528932139142333No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC31940.18580699807095805No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGA31420.18278196241044148No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30680.17847710397047564No Hit
TCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCC30460.17719728119102635No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG30310.17632467475049274No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA30040.17475398315753224No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT29690.1727179014629538No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC28560.16614426627760054No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC28500.16579522370138708No Hit
GTCAGGGACCCCGGACTGCCGGGTAGATGCCCAGTAAATGAGTAGGTTAG27760.16149036526142124No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT27460.15974515238035403No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC27330.15898889346522488No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA26950.15677829048253974No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA26860.15625472661821954No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA26840.15613837909281508No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG26310.1530551696695963No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG26120.1519498681782537No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC25100.14601614438262514No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT24640.14334015129832203No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC24570.14293293495940634No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA24440.14217667604427722No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA24370.14176945970536153No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA24260.14112954831563687No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC24130.14037328940050775No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT24040.13984972553618757No Hit
ATCGATCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGTGG23680.1377554700789069No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGGTGAAGCTG23500.13670834235026655No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.13630112601135086No Hit
CGACTGGAGATGAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC23310.13560304085892397No Hit
CTCCTAACCTACTCATTTACTGGGCATCTACCCGGCAGTCCGGGGTCCCT23090.13432321807947467No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG22960.13356695916434555No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22770.13246165767300294No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC22740.13228713638489623No Hit
GTTTTATACAGCTTCACCAATAAGAACTACTTAGCTTGGTATCAGCAGAA22610.13153087746976708No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA21870.12722601902980124No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21780.1267024551654811No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT21490.1250154160471161No Hit
CTATCGATCACCTTCGGCCAAGGGACACGACTGGAGATGAAACGAACTGT21370.12431733089468919No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20890.12152499028498162No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG20840.12123412147147042No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGTCGTGTCCCTTGGCCGAAGGTG20810.1210596001833637No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA20520.1193725610649987No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20390.11861630214986957No Hit
AACCAGGACAGCCTCCTAACCTACTCATTTACTGGGCATCTACCCGGCAG19740.11483500757422391No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG19100.11111188676128046No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18770.10919215259210652No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA18650.10849406743967961No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT18640.10843589367697738No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG18560.10797050357535945No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18320.10657433327050567No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT17940.10436373028782049No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17800.10354929760998913No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17650.10267669116945553No Hit
TTATAGTACTCTATCGATCACCTTCGGCCAAGGGACACGACTGGAGATGA17540.10203677977973086No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC17300.10064060947487707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)