FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20466
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT248112.122544708296687No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT7243.5375745138278116TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCT6413.1320238444249RNA PCR Primer, Index 11 (96% over 25bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2401.1726766344180592No Hit
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCT2171.0602951236196618TruSeq Adapter, Index 11 (96% over 28bp)
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.9479136128212646No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.9039382390305873No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTG1840.8990520863871787TruSeq Adapter, Index 11 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC1680.8208736440926414TruSeq Adapter, Index 11 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTG1310.640085996286524TruSeq Adapter, Index 11 (96% over 26bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.5912244698524383No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.5179321802013095No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT1040.5081598749144923RNA PCR Primer, Index 11 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG870.42509527997654645No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC770.3762337535424607No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGCTTGA710.3469168376820092RNA PCR Primer, Index 11 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACACCTCGTATGCCGT590.28828300596110623No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.2638522427440633No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC500.244307632170429No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAACTCGTATGCCGT470.22964917424020326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT430.21010456366656896No Hit
GTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGGAGCTA430.21010456366656896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT420.20521841102316035No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC410.20033225837975177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC400.1954461057363432No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA380.18567380044952606No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.18078764780611745No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACAGCTCGTATGCCGT350.1710153425193003No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT350.1710153425193003No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG340.16612918987589173No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC340.16612918987589173No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG300.1465845793022574No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC300.1465845793022574No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT300.1465845793022574No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA300.1465845793022574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG270.13192612137203166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC250.1221538160852145No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTCTGC250.1221538160852145Illumina PCR Primer Index 11 (100% over 26bp)
CAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT230.11238151079839734No Hit
GCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG230.11238151079839734No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA230.11238151079839734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG230.11238151079839734No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG220.10749535815498877No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC210.10260920551158018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)