FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001963587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20466
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTG3481.7003811199061858No Hit
ATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTC1300.6351998436431154No Hit
CATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGTCGCCGT1070.5228183328447181Illumina Single End PCR Primer 1 (96% over 25bp)
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.3664614482556435No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.31271376917814914No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.23942147952702042No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT410.20033225837975177No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA400.1954461057363432No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC370.18078764780611745No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC350.1710153425193003No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.15635688458907457No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT310.151470731945666No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC300.1465845793022574No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC290.14169842665884883No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG270.13192612137203166No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.13192612137203166No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT250.1221538160852145No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC240.11726766344180592No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG230.11238151079839734No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC230.11238151079839734No Hit
TATACACATCTGACGCTGCCGACGATCGCATAAGTGTAGATCTCGGTGGT210.10260920551158018No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG210.10260920551158018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)