FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521676
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC25540.48957590535121415No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.4353276746486325No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.43091880784241565No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20950.4015902590880163No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20180.3868301397802467No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19390.3716866407501975No Hit
CTTCTATGTTGGTGCTCAGGTCCCGTGGGTGTTCGGCGGAGGGACCAAGT18800.3603769389429454No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18110.3471503385242948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA17830.34178302241237857No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCACGGGACCTGA17340.33239021921652523No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC17190.32951487129942725No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17080.3274062828268887No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15280.2929021078217131No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT14970.28695972212637727No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA14960.2867680322652374No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14380.2756500203191253No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14270.27354143184658675No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG14230.2727746724020273No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14150.2712411535129084No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC14130.27085777379062864No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13980.2679824258735307No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13930.2670239765678314No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT13820.26491538809529286No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13710.2628067996227544No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA13600.26069821115021585No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13040.24996357892638343No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT12710.24363781350876787No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA12460.23884556698027126No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12370.23712035823001248No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12230.23443670017405438No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT11670.223702067950222No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC11360.21775968225488618No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11250.21565109378234767No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC11200.21469264447664835No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11030.21143391683727067No Hit
GCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTTCTATGTTGGTGCTC10850.20798349933675306No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG10660.20434139197509563No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10590.20299956294711657No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10500.2012743541968578No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT10410.19954914544659902No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10300.19744055697406052No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10100.19360675975126324No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9920.19015634225074568No Hit
GGTCCCGTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGC9610.18421395655540987No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTG9600.18402226669427002No Hit
GTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC9590.18383057683313014No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG9440.18095522891603216No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9390.17999677961033284No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG9380.179805089749193No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA9260.1775048114155146No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT9140.17520453308183623No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA9060.17367101419271733No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC8890.17041228655333962No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8700.1667701791916822No Hit
GGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGGTGACTGCTCCAGT8540.16370314141344436No Hit
GTGCTTGACCAGGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGGTG8530.1635114515523045No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT8430.16159455294090583No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC8370.16044441377406665No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG8200.15718568613468897No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8140.15603554696784977No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT8140.15603554696784977No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT8080.1548854078010106No Hit
GAGTATTCCTGCCTGCTCTTCTATGTTGGTGCTCAGGTCCCGTGGGTGTT7920.15181837002277276No Hit
CTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTT7900.15143499030049304No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA7870.15085992071707344No Hit
CCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACA7810.14970978155023426No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC7760.14875133224453493No Hit
ACATAGAAGAGCAGGCAGGAATACTCAGCCTCGTCCTCAGGCTGCACACC7580.14530091474401738No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCACGGGACCTGAG7510.14395908571603833No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC7370.1412754276600802No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT7330.14050866821552074No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT7280.13955021890982142No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.13840007974298224No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG7220.13840007974298224No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG7200.1380167000207025No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG6980.13379952307562548No Hit
GGGTGCTTGACCAGGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGG6950.13322445349220588No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC6950.13322445349220588No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG6820.13073248529738765No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6800.13034910557510793No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG6730.12900727654712887No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6700.12843220696370927No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC6590.12632361849117077No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG6570.12594023876889104No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG6570.12594023876889104No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA6440.12344827057407279No Hit
GTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTTCT6430.12325658071293293No Hit
GTTTGCATAGTGACCATTGGTGACTGCTCCAGTGCTGGAAGCACAGGTGA6410.12287320099065319No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA6310.12095630237925455No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG6260.11999785307355523No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6230.11942278349013563No Hit
ATTGGTGACTGCTCCAGTGCTGGAAGCACAGGTGAGAGTGACTGTCCCTC6220.11923109362899577No Hit
CTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGA6160.11808095446215658No Hit
CTCCAGTGCTGGAAGCACAGGTGAGAGTGACTGTCCCTCCTGGGGACACA6080.11654743557303766No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA6080.11654743557303766No Hit
CCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTGC5990.11482222682277889No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC5980.11463053696163902No Hit
GTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCA5850.1121385687668208No Hit
GGTGAGAGTGACTGTCCCTCCTGGGGACACAGTCAGTGAGGGCTCCTGAG5820.1115634991834012No Hit
CCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAG5690.10907153098858297No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGT5670.10868815126630323No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.10792139182174378No Hit
GCAGCTACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTAC5600.10734632223832417No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA5600.10734632223832417No Hit
GGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTT5580.10696294251604445No Hit
GCCCTGGGTGCTTGACCAGGCTTCTGCTGGAACCAGTTTGCATAGTGACC5490.10523773376578564No Hit
GTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTGAGACCAAAATGG5460.10466266418236606No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC5450.10447097432122619No Hit
CAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA5390.103320835154387No Hit
AGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAG5360.10274576557096742No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC5360.10274576557096742No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT5340.10236238584868769Illumina PCR Primer Index 11 (95% over 22bp)
ACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCT5330.10217069598754781No Hit
AGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGT5320.10197900612640795No Hit
CTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGA5250.1006371770984289No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)