FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001963593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521676
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22140.42440135256366024No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18030.3456168196351759No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17440.3343071178279239No Hit
CTTCTATGTTGGTGCTCAGGTCCCGTGGGTGTTCGGCGGAGGGACCAAGT17250.33066501046626645No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC17160.3289398017160077No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC16390.31417968240823807No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA15530.29769435435020974No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15110.28964338018233543No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTG14860.28485113365383874No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCACGGGACCTGA14420.27641677976368473No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA14180.27181622309632797No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13970.26779073601239084No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13730.2631901793450341No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13490.2585896226776773No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCA13360.2560976544828591No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13010.24938850934296386No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12650.2424876743419287No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12580.24114584531394964No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT12440.23846218725799154No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12250.2348200798963341No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA12120.23232811170151588No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11900.22811093475643887No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11820.22657741586731994No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA10940.20970870808701184No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT10830.20760011961447333No Hit
GCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTTCTATGTTGGTGCTC10740.20587491086421456No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACC10710.20529984128079495No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10700.2051081514196551No Hit
TTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG10520.20165773391913755No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10460.20050759475229835No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10300.19744055697406052No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10130.19418182933468284No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG10040.19245662058442406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9820.18823944363934703No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC9610.18421395655540987No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9570.18344719711085042No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT9470.18153029849945176No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9320.17865495058235378No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9260.1775048114155146No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTG9220.17673805197095516No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG9200.17635467224867543No Hit
GAGTATTCCTGCCTGCTCTTCTATGTTGGTGCTCAGGTCCCGTGGGTGTT9170.17577960266525583No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.1748211533595565No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9120.1748211533595565No Hit
GTAGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC9090.1742460837761369No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG8700.1667701791916822No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.1652366603025633No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8530.1635114515523045No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.16006103405178693No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT8260.15833582530152815No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT8230.15776075571810855No Hit
GGTCCCGTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGC8050.154310338217591No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA7910.1516266801616329No Hit
CCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACA7880.1510516105782133No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA7850.1504765409947937No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG7790.1493264018279545No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT7750.14855964238339506No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC7650.14664274377199643No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC7630.14625936404971668No Hit
GTGCTTGACCAGGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGGTG7550.14472584516059778No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7510.14395908571603833No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC7440.14261725668805925No Hit
GGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGGTGACTGCTCCAGT7330.14050866821552074No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA7140.1368665608538633No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA7130.13667487099272346No Hit
CCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTG7040.13494966224246466No Hit
CTGTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTT7030.1347579723813248No Hit
ACATAGAAGAGCAGGCAGGAATACTCAGCCTCGTCCTCAGGCTGCACACC6910.13245769404764643No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC6890.1320743143253667No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG6730.12900727654712887No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC6660.12766544751914982No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG6610.1267069982134505No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT6520.12498178946319172No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC6430.12325658071293293No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCACGGGACCTGAG6420.12306489085179306No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT6420.12306489085179306No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC6250.11980616321241536No Hit
GGGTGCTTGACCAGGCTTCTGCTGGAACCAGTTTGCATAGTGACCATTGG6140.11769757473987685No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG6080.11654743557303766No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA6070.1163557457118978No Hit
GTCAGGTGTGCAGCCTGAGGACGAGGCTGAGTATTCCTGCCTGCTCTTCT6060.11616405585075794No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC6050.11597236598961808No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA6020.11539729640619849No Hit
GTTTGCATAGTGACCATTGGTGACTGCTCCAGTGCTGGAAGCACAGGTGA5960.1142471572393593No Hit
GTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCA5930.1136720876559397No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAG5800.11118011946112147No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA5690.10907153098858297No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG5620.10772970196060391No Hit
GGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGG5580.10696294251604445No Hit
CTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCA5560.10657956279376472No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA5510.1056211134880654No Hit
GCAGCTACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTAC5500.10542942362692553No Hit
CTCCAGTGCTGGAAGCACAGGTGAGAGTGACTGTCCCTCCTGGGGACACA5480.10504604390464578No Hit
CTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGA5440.10427928446008633No Hit
CCCTCCTTGGGGGCAAAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAG5400.10351252501552687No Hit
ATTGGTGACTGCTCCAGTGCTGGAAGCACAGGTGAGAGTGACTGTCCCTC5350.10255407570982755No Hit
GGTGAGAGTGACTGTCCCTCCTGGGGACACAGTCAGTGAGGGCTCCTGAG5340.10236238584868769No Hit
CCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGT5310.10178731626526809No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA5300.10159562640412823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)