FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001963601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001963601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704490
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT154210.5701999267884148No Hit
GGCTAACAGTTTCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCA151790.5612518441554599No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC136430.5044574023198459No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA131400.485858701640605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC127870.4728063331718734No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC119550.44204267717758244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT117940.43608961393830264No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT117690.43516522523655105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC106530.39390051359036266No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG105690.39079456755247755No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG105480.3900180810430062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT104420.38609867294757977No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA99050.36624280363395684No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCT98000.36236037108660046No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT95390.35270975304031443No Hit
GATCTATGCTGCATCCAATTTGGCCAGTGGGGTCCCATCAAGGTTCAGCG95060.3514895599540024No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC93030.34398352369578No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG90960.3363295852452773No Hit
GGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCC88030.32549574966074935No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAA86440.3196166375176096No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG83720.3095592884425529No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC78630.29073873447489174No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC77280.28574703548543345No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC77120.2851554267163125No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC76540.28301084492824896No Hit
GTGGGGTCCCATCAAGGTTCAGCGCCAGTGGATCTGGGACAGATTTCACT75760.2801267521787842No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT72300.26733321254654296No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG71340.26378355993181707No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA65720.24300330191644265No Hit
ATTGTCAACAGGCTAACAGTTTCCCTCGAACGTTCGGCCAAGGGACCAAG64810.23963852704206706No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC64690.23919482046522636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA63760.23575609449471066No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG61590.22773240056350735No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAAC61380.22695591405403606No Hit
GTCCCAGATCCACTGGCGCTGAACCTTGATGGGACCCCACTGGCCAAATT60410.2233692858912401No Hit
GAGTGAAATCTGTCCCAGATCCACTGGCGCTGAACCTTGATGGGACCCCA58770.21730529600775006No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC58490.21626998066178837No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT56640.20942950426882703No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA54460.20136883478955367No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA54300.2007772260204327No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT53600.19818893765552842No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC53390.19741245114605713No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA52980.19589645367518463No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC52050.1924577277046689No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG51940.19205099667589823No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT51730.19127451016642694No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG51640.1909417302337964No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA51300.18968456159941433No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA51130.18905597728222326No Hit
CTCCTGATCTATGCTGCATCCAATTTGGCCAGTGGGGTCCCATCAAGGTT51130.18905597728222326No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGGCGCTGAACCTTG50850.18802066193626155No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT50750.18765090645556093No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50230.18572817795591776No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA50220.1856912024078477No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC49620.18347266952364402No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC49580.18332476733136377No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT49570.1832877917832937No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC49200.18191969650470144No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC49190.18188272095663138No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC48270.17848097053418574No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC47670.17626243764998206No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC46400.17156654304508429No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA46220.1709009831798232No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTG45610.16864547474754946No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA45590.16857152365140932No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGT45480.16816479262263864No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG45310.16753620830544763No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGGAAACTGTTAGCCTGTTGACAA44950.1662050885749254No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44840.16579835754615474No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC44460.1643932867194924No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT44230.163542849113881No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT44140.16321006918125044No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC44110.16309914253704025No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC44060.16291426479668994No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG43330.16021504978757548No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCATCTGGATGT43210.15977134321073475No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC42210.1560737884037286No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.15163672263532127No Hit
ATGCTGCATCCAATTTGGCCAGTGGGGTCCCATCAAGGTTCAGCGCCAGT40630.15023165180865894No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT39990.147865216732175No Hit
GCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTTCGAGG39900.14753243679954445No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA39860.14738453460726422No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG39650.14660804809779293No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC39110.14461136850200962No Hit
AGTCAGGGTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAA38860.14368697980025807No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGT38160.14109869143535378No Hit
GGACCCCACTGGCCAAATTGGATGCAGCATAGATCAGGAGCTTAGGGGCT37770.13965664506062142No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT37680.13932386512799086No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT37440.1384364519743094No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37240.13769694101290816No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGGC36920.1365137234746662No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA36430.1347019216192332No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA36220.1339254351097619No Hit
GTATTAGCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT36080.13340777743678103No Hit
CTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA36000.13311197305222056No Hit
GCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGC35990.1330749975041505No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA35210.13019090475468573No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC35140.1299320759181953No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG34910.1290816383125839No Hit
GTTCAGCGCCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCC34680.1282312007069725No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT34650.1281202740627623No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC34520.1276395919378515No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCA34370.12708495871680056No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG34360.1270479831687305No Hit
CTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGC33780.12490340138066695No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC33290.12309159952523396No Hit
ACCTTGATGGGACCCCACTGGCCAAATTGGATGCAGCATAGATCAGGAGC33000.12201930863120218No Hit
ACTTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACGTTCGGCCAAGG32960.12187140643892196No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCC32830.12139072431401116No Hit
GCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATGCTGCATCCAATT32700.12091004218910036No Hit
CATCAAGGTTCAGCGCCAGTGGATCTGGGACAGATTTCACTCTCACCATC32430.11991170239120869No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC32370.11968984910278833No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT31770.11747131621858466No Hit
ATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA31610.11687970744946366No Hit
GTCCCTTGGCCGAACGTTCGAGGGAAACTGTTAGCCTGTTGACAATAGTA31320.1158074165554319No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCAATTTGGCCAGTGGGGTCCCA31060.1148460523056103No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT30940.11440234572876956No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG30920.11432839463262943No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT30590.11310820154631741No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC30440.1125535683252665No Hit
TTACTATTGTCAACAGGCTAACAGTTTCCCTCGAACGTTCGGCCAAGGGA30360.11225776394070601No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG30320.11210986174842576No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC30060.11114849749860418No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA29970.11081571756597361No Hit
GGCCAGTGGGGTCCCATCAAGGTTCAGCGCCAGTGGATCTGGGACAGATT29940.11070479092176344No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC29930.11066781537369338No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29870.110445962085273No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT29630.10955854893159153No Hit
GCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAA29370.10859718468176996No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGTTGCTA29300.10833835584527951No Hit
CACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAACTGGTTAGCCTGGT29230.10807952700878909No Hit
GGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTCTCCATCTTCC29230.10807952700878909No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA29220.10804255146071903No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG29200.1079686003645789No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT28830.10660050508598663No Hit
CAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGT28780.10641562734563634No Hit
CTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGATGACCCAGTC28770.10637865179756627No Hit
ACCCAGTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCAT28250.10445592329792308No Hit
CCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAG28040.10367943678845179No Hit
GGCTAACCAGTTGCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTC27970.10342060795196137No Hit
GGCCAAATTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT27820.10286597473091044No Hit
GGTTTCTGCTGATACCAGGCTAACCAGTTGCTAATACCCTGACTCGCCCG27770.10268109699056013No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT27700.10242226815406971No Hit
ACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAA27620.10212646376950923No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG27620.10212646376950923No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG27580.10197856157722898No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC27110.10024071081793609No Hit
ACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAG27100.10020373526986603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)