FastQCFastQC Report
Sun 14 May 2023
EGAF00001975191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54664605
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC11361702.0784381410969677TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC3390990.6203264434088567TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC1015090.18569419828424627TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC552000.1009794180347594TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)