FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001975204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19101623
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC4186232.1915572305033977TruSeq Adapter, Index 5 (100% over 51bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2025221.0602345151508854Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGA1634050.8554508692795372TruSeq Adapter, Index 5 (100% over 40bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA1543290.8079365821427844TruSeq Adapter, Index 5 (100% over 36bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA974930.5103911850841156Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT840660.44009872878341283TruSeq Adapter, Index 5 (100% over 38bp)
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC779620.40814332897262184TruSeq Adapter, Index 5 (100% over 42bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG632270.3310032869981781Illumina Single End PCR Primer 1 (96% over 30bp)
TATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG278830.14597188940437156TruSeq Adapter, Index 5 (100% over 39bp)
ATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG269020.14083620014906587TruSeq Adapter, Index 5 (100% over 39bp)
TATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG192180.10060925189445945TruSeq Adapter, Index 5 (100% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)