FastQCFastQC Report
Sat 1 Apr 2023
EGAF00001975213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36478888
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC26628727.299761988358856TruSeq Adapter, Index 4 (100% over 51bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG4215321.1555505748969104Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)