FastQCFastQC Report
Thu 2 May 2024
EGAF00001975350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39887024
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG27772366.96275560693623Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1449550.3634139262934232No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT1420470.35612333474665847Illumina Paired End Adapter 2 (100% over 30bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT494400.1239500846189979Illumina Single End PCR Primer 1 (100% over 31bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT467120.11711076765215675Illumina Paired End Adapter 2 (96% over 30bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG464730.11651157529325828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)