FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001975350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39887024
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA6417961.6090345572033653Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1397440.3503495272046368Illumina Single End PCR Primer 1 (100% over 45bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1219330.3056959075212029No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG950730.238355711872613Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAC751980.1885274770060559Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA687380.17233173374880012Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAACA639610.1603554078138294Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAA519280.130187702145941Illumina Single End PCR Primer 1 (100% over 57bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA488610.12249848472024386Illumina Single End PCR Primer 1 (100% over 56bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAACAA465320.11665949307223321Illumina Single End PCR Primer 1 (100% over 57bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG454280.11389167564870219Illumina Single End PCR Primer 1 (100% over 45bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGG447370.11215928267799573No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAT422730.10598183509504244Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)