FastQCFastQC Report
Sun 12 May 2024
EGAF00001975372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48543785
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGAGAAATCTCGTA2057250.42379266470465793Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG874710.18018990484569755Illumina Paired End PCR Primer 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)