FastQCFastQC Report
Wed 1 May 2024
EGAF00001975390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45250905
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTA2250193549.727038608399106Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG27497636.076702775336758Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA12380742.736020417713193Illumina Paired End Adapter 2 (100% over 32bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG3532740.7807004080912856Illumina Paired End PCR Primer 2 (100% over 31bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2546750.5628064234295425No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC2029340.44846395889761764Illumina Paired End Adapter 2 (96% over 28bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTAT1035920.22892801812472038Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCAGCTCGTA1016490.2246341813495222Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCCGGAATGCCGAGACCGAGTTCCATCTCGTA939450.20760910748635858Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCACCTCGTA664540.14685673137365982Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATATCGTA621580.13736299859638165Illumina Paired End PCR Primer 2 (97% over 37bp)
GATGGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTA547920.12108487112025715Illumina Paired End PCR Primer 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)