FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001975390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45250905
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2220975449.08134765481486Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCAT24967245.517511749212529Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCA11603502.5642581071030515Illumina Paired End PCR Primer 2 (97% over 38bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTATGCCG3170280.7006003526338314Illumina Paired End PCR Primer 2 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1888760.4173971769183401No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATC1814660.4010218138178673Illumina Paired End PCR Primer 2 (97% over 38bp)
ATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA958820.21188968485823653Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCCGGAATGCCGAGACCGAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA917100.2026699797495763Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA659130.14566117517428656Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCAA624210.13794420244191802Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATATCGTATGCCGTCTTCTGCTTGAAAAAAAAA519220.11474245653208483Illumina Paired End PCR Primer 2 (97% over 37bp)
GATGGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA495790.10956465953553857Illumina Paired End PCR Primer 2 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)