FastQCFastQC Report
Thu 2 May 2024
EGAF00001975395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46668918
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAATGATCTCGTA8285891.775462203773398Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1738740.3725691690559443No Hit
GCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCT954690.20456655969611293No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT777110.16651553824324788No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG668070.14315095113197182Illumina Paired End PCR Primer 2 (97% over 36bp)
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG649820.1392404255011869No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)