FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001975395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46668918
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA8080031.7313514746581442Illumina Paired End PCR Primer 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1224700.2624230542477972No Hit
GCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCTGTCCGGGAGGGACCACCGGGCCGCG907690.1944956169757353No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCA737840.15810094418730686No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCGCCGAGGGACGCCTGGGGAAGGGAGG616870.1321800518280711No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGAATGAT604910.12961731831880055Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)