FastQCFastQC Report
Fri 10 May 2024
EGAF00001975413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20599141
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3701391.7968661897115028Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT797740.3872685759080925Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT211090.10247514690054307Illumina Paired End Adapter 2 (96% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)