FastQCFastQC Report
Wed 8 May 2024
EGAF00001975441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928364
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG117348011.819469954969419Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT2965152.986544409532124Illumina Paired End Adapter 2 (100% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA372360.37504668442857253Illumina Paired End Adapter 2 (96% over 29bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT369600.3722667702352573Illumina Paired End Adapter 2 (96% over 30bp)
CGGCAGTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT321530.3238499313683503Illumina Paired End Adapter 2 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)