FastQCFastQC Report
Fri 3 May 2024
EGAF00001975464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18282620
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG7740814.233971936188577Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT1635070.8943302436959254Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT212990.11649862000085327Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA196610.10753929141446904Illumina Paired End Adapter 2 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)