FastQCFastQC Report
Wed 8 May 2024
EGAF00001975471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15283459
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCCAGATCTCGTA1317940.862330968401852Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG469310.3070705394636123Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA238860.15628661024968235Illumina Paired End Adapter 2 (100% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)