FastQCFastQC Report
Wed 8 May 2024
EGAF00001975472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15283459
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1549431.0137953718461248Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN745130.4875401569762447No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT214850.14057681575878864Illumina Paired End Adapter 2 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)