FastQCFastQC Report
Thu 9 May 2024
EGAF00001975475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5004483
Total Bases625.5 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG58899711.769387567107332Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT1315982.629602298579094Illumina Paired End Adapter 2 (100% over 30bp)
GGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT185540.37074758771285665Illumina Paired End Adapter 2 (96% over 30bp)
CGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTA182180.3640336074675446Illumina Paired End Adapter 2 (96% over 29bp)
CGGCAGTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGT149080.29789290921759554Illumina Paired End Adapter 2 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)