FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001975487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45591395
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA36353037.973660380429245Illumina Paired End PCR Primer 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10383202.2774473121517778No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTAT1679900.3684686551047626Illumina Paired End PCR Primer 2 (97% over 37bp)
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT882760.19362425738453495No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTA826350.1812513084980181Illumina Paired End PCR Primer 2 (97% over 37bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATGCTATCTCGTATGCCG796330.174666732614784Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)