FastQCFastQC Report
Sun 2 Apr 2023
EGAF00001975511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32068263
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA4232761.3199218180292458Illumina Single End PCR Primer 1 (100% over 57bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA817840.2550309631675404No Hit
CGGCATTCCTGCTGAACCGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG658550.205358799757879Illumina Single End PCR Primer 1 (100% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAG610180.1902753510534699Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAC499710.1558269620028999Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGA445960.13906584213806653Illumina Single End PCR Primer 1 (100% over 57bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAACA431870.13467208997256883Illumina Single End PCR Primer 1 (100% over 57bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGG360320.11236031087807904No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAGAA340950.1063200710309754Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)