FastQCFastQC Report
Fri 10 May 2024
EGAF00001975513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51265583
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTA6687551.3044911632039764Illumina Paired End Sequencing Primer 2 (100% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590280.3102042163453013No Hit
GCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCT838560.16357172803438128No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG640940.12502344896770215No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG548380.10696845093910275Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)