FastQCFastQC Report
Sat 13 May 2023
EGAF00001975513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51265583
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6513161.2704741892821154Illumina Paired End PCR Primer 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1113110.21712617605460569No Hit
GCGACCGGCAAGGCGGAGGTCGACCCACGCCACACGTCGCACGAACGCCTGTCCGGGAGGGACCACCGGGCCGCG802940.15662359677056634No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCGCCGAGGGACGCCTGGGGAAGGGAGG612990.11957144815850432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)