FastQCFastQC Report
Tue 14 May 2024
EGAF00001975534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65733519
Total Bases8.2 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTATATCTCGTA2986200.4542887776934626Illumina Paired End PCR Primer 2 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)