FastQCFastQC Report
Wed 22 May 2024
EGAF00001975547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94768559
Total Bases11.8 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG15799921.6672111686324154Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2141840.22600744620375626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)