FastQCFastQC Report
Sat 25 May 2024
EGAF00001975548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94768559
Total Bases11.8 Gbp
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAAAGATCTCGTA15489871.6344946217869578Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG1364120.14394225409716319Illumina Paired End PCR Primer 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)