FastQCFastQC Report
Wed 8 May 2024
EGAF00001975557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001975557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14707563
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTTCCATCTCGTA4069642.767038971718156Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG2373011.6134624070622712Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA904050.6146837514821456Illumina Paired End Adapter 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC165330.11241155315805887Illumina Paired End Adapter 2 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)