FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001982491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001982491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10623315
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1961591.846495185354101No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA801610.7545761374862743No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA689750.6492794386686265No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA678750.6389248553770644No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC658060.6194488255313901No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT638830.6013471312862322No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG493090.4641583159305735No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG396390.3731321155402057No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC382010.35959585120087284No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG372330.3504838179042982No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG339120.31922238962131877No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG256280.2412429641783191No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT251650.23688462593832527No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC226910.21359622678984855No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT199870.1881427784076816No Hit
GACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAA196370.1848481382694573No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA187090.1761126352743941No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC184130.17332631104321014No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA172010.16191744290741636No Hit
CAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACC171970.16187978987726523No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA167930.15807683383200066No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC157060.14784462288842984No Hit
CTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTTAAACTATT136920.1288863222073336No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA132650.12486686123869997No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC130670.1230030362462188No Hit
ACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAA129020.12144984875248452No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG128780.12122393057157771No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG124820.11749628058661539No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA123560.11631021013685464No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC122920.11570776165443648No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA122860.11565128210920979No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG120390.11332620749737723Illumina Single End PCR Primer 1 (100% over 50bp)
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC119060.112074244244852No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC118080.1111517450061492No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG111900.10533435184779892No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC110590.10410121511034925No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA107770.10144667648469428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)