FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001982493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001982493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10659995
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1982741.8599821106857932No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA814630.7641936042183886No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA702050.6585837985852714No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA683280.6409759104014588No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC667560.6262291867866729No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT649930.6096907174909557No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG504450.47321785798210975No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG405740.3806193154874838No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC385990.3620921022946071No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG378080.35467183615001696No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG343640.3223641286886157No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG261520.24532844527600622No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT253080.23741099315712624No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC232610.21820835750861045No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT201570.18909014497661586No Hit
GACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAA198280.18600383958904296No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA193140.18118207372517528No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC185080.1736210945689937No Hit
CAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACC171320.16071302097233628No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA170620.1600563602515761No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA168510.1580769972218561No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC158480.14866798718010657No Hit
CTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTTAAACTATT137970.12942782806183306No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA135830.12742032242979476No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC133410.1251501525094524No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG129370.12136028206392217No Hit
ACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAA127350.11946534684115706No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA127240.11936215729932331No Hit
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG125930.11813326366475782No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA125490.11772050549742284No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG125220.11746722207655819Illumina Single End PCR Primer 1 (100% over 50bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC122010.11445596362850076No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC120090.11265483708012997No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC118650.11130399216885187No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC113110.10610699160740694No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG112990.10599442119813376No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA108250.10154789003184335No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT108160.10146346222488847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)