FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001982495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001982495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10683704
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT1986231.8591211437531403No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA809170.7573871383932015No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA700000.6552034762475636No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA685460.6415939640409356No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC670850.6279189314866829No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT648350.6068588197501541No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG502480.47032377534982245No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG404520.37863272887380633No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC385940.36124175660426383No Hit
CAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAG375900.3518442667449417No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG343840.3218359475328032No Hit
CCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGG262740.24592594478469262No Hit
CTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAAT252720.2365471750246918No Hit
GCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGC231770.2169378709855683No Hit
ACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGT202420.18946612523147402No Hit
GACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAA199270.18651770958835998No Hit
CCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCA193010.18065831850077463No Hit
CGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAATGGGAC184880.17304859812664222No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA172730.16167613778891665No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA169790.1589242831886769No Hit
CAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACC169380.15854052115258904No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC158590.14844102756871586No Hit
CTTTAACTCCACCATTAGCACCCAAAGCTAAGATTCTAATTTAAACTATT137460.12866324263570014No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA134910.12627642997222688No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC134120.12553698604903318No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG130760.12239200936304487No Hit
ACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCTTACTAGACCAA127340.1191908723790925No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG126840.11872286989605851Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAG126830.11871350984639785No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA126440.11834846790963133No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC124010.11607397584208622No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA123840.11591485499785467No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC121260.11349996218539937No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC120620.1129009190071159No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG113350.1060961629038019No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC112030.1048606363485922No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA108810.10184670035785343No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT108070.10115405668296314No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA106990.10014317131960976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)