FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001982653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001982653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9933842
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT523940.5274293672075718No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG520500.5239664572881269No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT494910.4982060314629526No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA338770.3410261608751176No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC324450.3266107916755672No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG293470.2954244692033555No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG274360.27618719927295No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT211880.2132910912011687No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT205090.2064558707497059No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA203240.2045935500081439No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC201950.20329495878835196No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA198360.19968104988986132No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA196020.19732546581675048No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG185810.1870474686430487No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA173200.1743534878046178No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC162830.1639144250532674No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA156790.15783419949703248No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC155260.15629400991076767No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG147020.14799913266186435No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC143620.14457648913683144No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT141750.14269403519806334No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT140940.14187864071121728No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT135800.13670440902925574No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA131170.13204357387604915No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG128550.1294061250420532No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA119140.11993345575659449No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA118180.11896706229070284No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGC110100.11083325061944814No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT108940.10966552518149573No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT104500.10519595540174687No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC104150.10484362445064054No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG103070.10375643180151244No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC100750.10142098092560763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)