FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001982655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001982655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9972105
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG522130.523590555855559No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT521890.5233498845028206No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT497340.49873121071228194No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA335270.3362078518026034No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC321290.32218874550558785No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG296670.29749987590383375No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG277640.2784166432262797No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT212360.21295403528141751No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT206600.20717792281569436No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA203110.20367816022795587No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC202960.20352774063249435No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA198700.1992558241213866No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA196470.19701958613552506No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG186880.18740275999901726No Hit
ATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCA175240.17573019939120174No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC161290.16174117701327856No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA156660.15709822550003236No Hit
CCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTC154500.15493218332538616No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG149070.1494869939696784No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC146170.14657888179075532No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT141610.1420061260887245No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT141290.14168523095173988No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT136620.1370021675463706No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA132090.13245949576343208No Hit
CACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCG127590.1279469078995859No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA121480.12181981637778584No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA117700.11802924257215504No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT112020.11233335389067806No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGC109240.10954557738812418No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT105240.10553438817581644No Hit
CCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGC104100.10439119925030874No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC101800.1020847654532318No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG101460.10174381437018562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)