FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7306343
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA689570.9437963698118198No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA608710.8331254089768302No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC545630.7467894677268778No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG406180.55592791085773Illumina Single End PCR Primer 1 (100% over 50bp)
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC307960.4214967734200269No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT294450.40300599082194744No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG228510.3127556425971242No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA220720.30209367394878667No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC206690.28289118099163973No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA204250.2795516169990924No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA200250.27407692192934274No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT193990.2655090241451845No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG190650.26093765376194356No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT185000.25320464697592215No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA184100.25197284058522845No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC178820.2447462430931589No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG175650.24040754725038227No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG155750.2131709392783777No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA155100.21228130132954337No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC154160.2109947479881522No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG153250.20974925485978416No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC148980.20390501787282642No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT147240.20152352551748529No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG145340.19892304535935418No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT131680.18022696169615907No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA126790.17353414697339012No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT126310.17287718356502013No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT118940.16279005789900639No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG115740.15841030184320665No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA115350.15787651907390607No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG114340.15649415856879426No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA113230.15497493068693874No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT105850.14487411828325059No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC105400.14425821508790376No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG105230.1440255405474394No Hit
CACTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTA105040.1437654925316263No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG104630.14320433628697693No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA104430.14293060153348947No Hit
GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA103990.14232838507581702No Hit
CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT103070.14106920520977456No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG101840.13938573647582655No Hit
AGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGC100070.13696318390746232No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA94860.1298323935791134No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGG94420.12923017712144091No Hit
GGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGA93200.12756039512516726No Hit
CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG90900.12441244546006121No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC89540.12255104913634632No Hit
CCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAACGATG88900.12167509792518638No Hit
CAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAG85760.11737746229543287No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA83160.1138189105000956No Hit
AGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGAGGCG82230.1125460438963788No Hit
CTTGTGGCTGGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTC82000.1122312489298682No Hit
CTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGT81660.11176589984893948No Hit
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCC81520.11157428552149824No Hit
GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT80940.11078045473638454No Hit
CTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTACC80440.11009611785266582No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT78780.10782411939871972No Hit
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAG77620.10623645782849231No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC76650.10490884427407801No Hit
CACGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGC73720.10089863013548639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)