FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7284043
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC768091.0544830666156144No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG462100.6344004284433796No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA427440.5868169641502665No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT408680.5610620365640345TruSeq Adapter, Index 27 (97% over 44bp)
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC368360.5057081623488494No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG345280.47402246252527613No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA329220.4519742675873824No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT303280.41636217688445826No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT268200.36820210973493706No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA213110.2925710350693976No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA211570.29045682459590094No Hit
CCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGT186210.25564099498039755No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA177170.2432303049281834No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT175880.241459310440644No Hit
GCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGG161340.2214978686973704No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC152560.20944412327055179No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA151760.2083458321154886No Hit
CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC147760.20285437634017262No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG144980.199037814576328No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG143610.19715699097328226No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG141690.1945210922011306No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG137520.1887962495553637No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT137060.18816473214120236No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC136030.1867506822790585No Hit
CCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACCGACGC134960.18528171785916148No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG132210.18150634201363172No Hit
GGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCT128630.1765914890947239No Hit
CCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC126550.1737359320915596No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA123110.16901328012478783No Hit
CACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTTACCGT114450.15712427837122872No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC114080.15661631871201198No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC101800.13975754948179192No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG99130.13609200275176847No Hit
GTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAG97320.13360711901343802No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC97030.1332089884697276No Hit
GCCAGTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAG95460.13105359207791606No Hit
CCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTACCGACGCT94640.12992784364397628No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA93120.1278410904493562No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG92780.12737431670845437No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA91360.12542484990821717No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC89780.12325572487696737No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC88170.12104541392740269No Hit
GCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACC88120.12097677073021124No Hit
CCCCAGTCATGAATCACAAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTA87890.12066101202313056No Hit
CCCCTCTTTGGTCTTGCGACGTTATGCGGTATTAGCTACCGTTTCCAGTA87830.12057864018650082No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT87170.11967254998357368No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG84870.116514962912767No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT83870.115142098968938No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT83440.11455176747309152No Hit
GCCCGGCCAACATAGCCTTCTCCGTCCCCCCTTCGCAGTAACACCAAGTA80590.11063910523317888No Hit
CTCAGGGTTTGTTATAATTTTTTATTTTTATGGGCTTTGGTGAGGGAGGT79970.10978792958800489No Hit
CTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATGTCAGCAT79700.10941725632317106No Hit
CCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGT76660.10524374993393093No Hit
CTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGGCTAGATCACCGG74810.10270395163784728No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC74400.10214107742087739No Hit
GGGTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGG74170.10182531871379673No Hit
CCCCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTC73420.10079567075592497No Hit
CCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACAC73090.1003426256544614No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)