FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7284043
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA683660.9385721638381321No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA606090.8320791077153169No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC543740.7464810408175789No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG405710.5569846306508625Illumina Single End PCR Primer 1 (100% over 50bp)
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC303540.4167191215098538No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT298170.4093468421314921No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG229230.314701601843921No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA222680.3057093430118411No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC210160.28852108643510205No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA204260.28042118916651093No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA203220.2789934106649288No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT196460.26971285040464477No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG191640.26309564619538905No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT184870.25380135729566666No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA179810.24685466573989198No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC178530.24509739989179088No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG174530.23960594411647487No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG155330.2132469563949581No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG152150.2088812490535819No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC151410.20786532973514849No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA151340.20776922925908042No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC149000.20455672763052055No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT148860.2043645266783845No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG146210.20072643722723768No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT132540.18195938711509527No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA126630.1738457612070659No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT124570.17101766148277817No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT119470.1640160553692503No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA114580.1573027506839265No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA114340.15697326333740752No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG114070.1566025900725737No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG112380.1542824500075027No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG106970.14685525607138783No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC106410.14608645226284359No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA105510.1448508747133975No Hit
CACTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTA105260.14450765872744026No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG104780.14384868403440232No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT104710.1437525835583343No Hit
CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT103450.14202277498910976No Hit
GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA103350.14188548859472686No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG102630.14089702655516997No Hit
AGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGC100980.13863180104785214No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA95640.1313007075878053No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGG94070.12914531119599376No Hit
GGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGA93580.12847260786351755No Hit
CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG91360.12542484990821717No Hit
CCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGGAAACGATG89060.12226726283741049No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC88690.12175930317819376No Hit
CAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAG85520.11740732447625583No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA83840.11510091305062312No Hit
AGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAGGCTGAGGCG82500.11326127536589227No Hit
CTTGTGGCTGGGGGTGAAAGGCCAATCAAACCGGGAGATAGCTGGTTCTC82050.11264348659116921No Hit
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCC80620.11068029115149376No Hit
CTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGT80520.11054300475711085No Hit
CTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTACC80220.11013114557396216No Hit
GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT79890.10967810047249858No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT79400.10900539714002239No Hit
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAG76070.10443376020707182No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC74880.10280005211391531No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT73210.10050736932772089No Hit
CACGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGC73020.10024652517839337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)