FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111435
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA782360.8586572806588644No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA569290.6248082766326051No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC484030.5312335543193799No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT451390.4954104375435922No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC357530.3923970263740015No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT266930.2929615367941493No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA245620.26957334382564324No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG245530.2694745668492394No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA234860.25776400753558576No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG230030.2524629764685804No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA221740.24336451941982792No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC220110.24157555862495866No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG215900.23695499117317964No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC213390.2342002110534729No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG212680.23342097046184274No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG209230.22963451969969603No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG208750.22910770915887563No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT200200.21972389640051213No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA196610.21578379256395946No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC192740.21153638257859492No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA186420.2046000437911262No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA179950.1974990767096511No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177470.1947772222487457No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT174660.19169318554102618No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG172670.18950911684054159No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG172180.18897133108012076No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA169730.1862824022780166No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG168810.18527268207477748No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG161660.17742540006047347Illumina Single End PCR Primer 1 (100% over 50bp)
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA160780.17645958073563606No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT159190.17471452081916844No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG151450.16621970084843934No Hit
GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA146150.160402834460214No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT145880.16010650353100253No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA138950.15250067634790787No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC138780.15231409761470063No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC138080.1515458322426709No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT137450.1508543934078441No Hit
CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT130380.1430949131503435No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT128010.14049378610504273No Hit
CACTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTA124550.1366963601232956No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA123430.13546713552804798No Hit
CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG122060.13396353044278975No Hit
CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA122010.13390865434478763No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGG112660.12364682401839008No Hit
AGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGC111220.12206639239592885No Hit
GGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGA109520.12020060506385656No Hit
CTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGT108200.11875187607660045No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA107560.11804946202217323No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA104010.1141532590640223No Hit
GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT103810.11393375467201379No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG103070.11312158842158232No Hit
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCC100980.11082776752509348No Hit
CTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTACC100930.11077289142709135No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT100670.1104875357174803No Hit
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAG100510.11031193220387349No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT100160.10992779951785861No Hit
GGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCT98260.10784250779377783No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC95940.10529625684647917No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT92210.10120249993552058No Hit
CGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGG91960.10092811944550995No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC91750.10069763983390104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)