FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9135916
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA793370.8684077217872843No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA577940.6326021386361258No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC489370.53565510015635No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT461840.5055212854408906No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC367170.401897302908652No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT274390.30034207845168454No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG249860.2734920067128463No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA248670.27218945533211997No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA241700.264560225816437No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG233410.2554861493910408No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA225460.246784230502995No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC222120.24312832998902353No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG221840.2428218473112056No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC218540.2392097300369224No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG216350.23681259766398904No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG214960.23529113008482128No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG212450.23254373179438162No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA202750.22192629616997356No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT201090.2201092917229099No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC199920.21882863196202768No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA188700.20654743322946492No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186770.20443489191450537No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA183260.20059291263185872No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT177500.19428812611674626No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA176540.1932373283642275No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG176000.19264625462843574No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG175180.19174869821482599No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG171390.18760023625436134No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG166880.1826636759795077Illumina Single End PCR Primer 1 (100% over 50bp)
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA161850.17715793358870638No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT161560.17684050510096633No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG156180.17095165936289256No Hit
GCGACTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAA151280.16558821250107816No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT151190.16548970021177953No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA143030.15655791931537022No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC140790.1541060578928265No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC140560.1538543042646189No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT140400.15367917130586578No Hit
CCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGAT134020.14669574457558499No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT130930.14331348930966528No Hit
CACTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTA127220.13925259382857724No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA124790.1365927620175142No Hit
CTTATATTCTGTAGCAAGGTTAACCGAATAGGGGAGCCGAAGGGAAACCG124340.136100200571021No Hit
CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA121950.13348415199964625No Hit
AGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGC115030.12590965153357364No Hit
GGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTA113340.12405980965674378No Hit
GATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGG111890.1224726672180436No Hit
CTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGT111260.12178308119295317No Hit
GGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGA110440.12088552477934342No Hit
GGTTAACCGAATAGGGGAGCCGAAGGGAAACCGAGTCTTAACTGGGCGTT105720.11571910249612628No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA105500.11547829467784074No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT104860.11477776284282826No Hit
CTGCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCC103570.11336575336288118No Hit
GTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACAG102660.1123696846599728No Hit
CTACTATAGCCACCCTAACCCTAACTTCCCTAATTCCCCCCATCCTTACC100330.10981931094813044No Hit
GCATCTGATACTGGCAAGCTTGAGTCTCGTAGAGGGGGGTAGAATTCCAG100010.10946904503062418No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGT99860.10930485788179314No Hit
GGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCT98850.10819933107966405No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC95950.10502504620226369No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC95130.10412748978865392No Hit
CGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGG94740.10370060320169319No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT93430.102266702101902No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT92790.10156617026688951No Hit
GGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATA91540.10019794402663072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)