FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001983391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10692917
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA637410.5961048795197793No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT388360.3631936916745917No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA356410.3333140994173994No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC319260.2985714749305545No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC270460.2529337878522764No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT211020.1973455886733246No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC203300.19012585620930192No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT188960.1767151096375292No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG174810.16348205078183997No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC171410.16030237586245175No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA168400.15748742836028748No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG168100.15720686880857673No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG164380.15372793036736374No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA163140.15256828422029273No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG161870.15138058211805067No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA158050.14780812382626743No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC152380.1425055482989347No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG151330.14152358986794716No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG148700.1390640177979498No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA146480.13698787711529042No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG142970.13370533036027493No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA142690.13344347477867827No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC139640.1305911193362859No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA139640.1305911193362859No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA135170.12641078201579606No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT128030.1197334646850808No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT125670.11752639621162309No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA125600.1174609323162239No Hit
GCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAA118780.11108287850733342No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT118620.11093324674642101No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG117410.1098016565545211No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG116200.10867006636262116No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC111840.10459260087775861No Hit
TGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTA110420.10326461899966116No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT109160.10208626888247613No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA107300.10034679966186963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)