FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001983537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11416470
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA681860.5972599236016037No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA455550.3990287715905179No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC373810.32743045792613656No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC312620.27383245434008935No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT243060.21290293759804912No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC223550.19581359211735325No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA213490.18700176149019793No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG212310.18596816704287752No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG209150.18320023615005337No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG205020.17958265558443198No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA201730.17670085411690303No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT195720.1714365298555508No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG190530.16689046614233646No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG188480.1650948147719917No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG183960.1611356224822559No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTA182850.16016334295977655No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC181360.15885821098816008No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA179490.15722022656740656No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGG176160.15430338799996848No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG172990.15152669783216705No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA172140.15078215945909726No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT171470.1501952880356187No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT167490.14670909659465667No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA161720.14165499493275943No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC161170.1411732348090084No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG159940.14009584398680153No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC146280.12813067436782122No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT143500.12569559592413418No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC143390.12559924389938396No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG143110.1253539842000198Illumina Single End PCR Primer 1 (100% over 50bp)
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT140720.12326051748044711No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC134510.11782100771954904No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG128990.11298588793208408No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA127550.11172455233535411No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA125870.1102529941391691No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT125310.10976247474044079No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT119410.10459450250383875No Hit
CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA118700.10397259398045106No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA116470.10201927566051504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)