FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001983538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11411800
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC720400.6312763981142326No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT559620.490387143132547No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG430260.37703079268827006No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA404850.3547643667081442No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC374700.32834434532676704No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG330400.28952487775811003No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA322630.2827161359294765No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA318900.2794475893373526No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG308260.27012390683327786No Hit
CTCCGTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTT261950.22954310450586235No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC223280.19565712683362835TruSeq Adapter, Index 12 (100% over 50bp)
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG211880.18566746700783401No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA209170.18329273208433375No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC206480.18093552287982614No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT191010.1673793792390333No Hit
GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCCATTGTAGCA181800.159308785642931No Hit
CTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCTAGGGATCGTCG179480.15727580223978688No Hit
CCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGT178350.15628559911670378No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC171120.14995005170087103No Hit
GCTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGG160870.14096812071715242No Hit
CCTTGGTCTTCCGGCGAGCGGGCTTTTCACCCGCTTTATCGTTACTTATG160820.14092430641967085No Hit
GTTGATTTCTTTTCCTCGGGGTACTTAGATGTTTCAGTTCCCCCGGTTCG156840.13743668834013914No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG152870.13395783312010373No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC151340.1326171156171682No Hit
GGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTT148220.12988310345431922No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA146580.12844599449692423No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA143030.12533517937573388No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGC132810.11637953697050422No Hit
CCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCC132230.11587129111971818No Hit
CTTTCTTTAAATGATGGCTGCTTCTAAGCCAACATCCTGGCTGTCTGGGC129980.11389964773304824No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG125500.10997388667870099No Hit
CCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTT125270.10977234091028584No Hit
CTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACA118770.10407648223768382No Hit
CCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCT117980.10338421633747524No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG116630.10220123030547329No Hit
GGGCATTTGTTGCTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCT116240.10185947878511716No Hit
CCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC114940.10072030705059676No Hit
GGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTAACTTTACTC114840.10063267845563365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)