FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001983541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001983541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11365757
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA684130.6019220717106657No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA453120.3986712015750469No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC375490.33036954775647587No Hit
CATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAC309910.27266991543106195No Hit
CTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGT243240.21401126207431673No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC224210.1972679866374057No Hit
CATGCACCGAAGCTGCGGCAGCGACGCTTATGCGTTGTTGGGTAGGGGAG212510.18697390767724492No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAA210650.1853374130733219No Hit
CCCGGTTTAAGCGTGTAGGCTGGTTTTCCAGGCAAATCCGGAAAATCAAG210180.18492389024329836No Hit
AAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGG205260.18059509806517948No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA196840.17318688055709797No Hit
CTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATT193760.17047698626673086No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAG193090.16988749627499516No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG185730.16341190472398803No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTA184270.16212734444348934No Hit
GGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCCGAAA182950.16096596117618914No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG182120.16023569745508373No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC182000.16013011715805642No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTACTATTCGG176130.15496548096180482No Hit
CGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATG173870.15297705203445752No Hit
GTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAA173390.15255473084634838No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT169980.14955449073915622No Hit
GGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGAT168120.14791799613523324No Hit
CTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCA163800.1441171054422508No Hit
CGAAGGGAAACCGAGTCTTAACTGGGCGTTAAGTTGCAGGGTATAGACCC160480.14119605055782908No Hit
CTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTG156930.13807263343743845No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC148880.1309899551785244No Hit
CGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGT143430.12619485002186834No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC143200.12599248778589936No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG143090.12589570584695767Illumina Single End PCR Primer 1 (100% over 50bp)
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGT138550.12190125127609186No Hit
CAAGGCTGAGGCGTGATGACGAGGCACTACGGTGCTGAAGCAACAAATGC133730.11766044267882904No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA130490.11480977465909221No Hit
GCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAG128240.11283014408983053No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT125300.11024342681266192No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA124330.109389986078358No Hit
CGAAGGTTGGCTAATCCTGGTCGGACATCAGGAGGTTAGTGCAATGGCAT119450.10509638733258153No Hit
CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTA118640.10438372032764733No Hit
CCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA115900.10197297021219089No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)